ABSTRACT
Objective To study the effect of graphene quantumdots (GQDs) on intestinal image formation and mesenteric microcirculation in mice. Methods The effect of GQDs on intestinal image formation was observed using FX-zn vivo imaging system in C57BL/6N mice by comparing with X-ray barium meal examination. Pure water was used as negative control and CdTe QDs was used as positivecontrol. The effects of GQDs on the mesenteric arterioles, venules, and capillaries were observed by the mesenteric microcirculation microscope. Results The mouse intestine took on a bright green image under ultraviolet stimulation 1 h after receivinggavage with GQDs, with clear edge and mucosal structure; meanwhile, mesenteric vesselswere in clear light green in the mice. High-density images of the small intestine were observed by X-ray in barium meal examination, but the intestinal mucosal structure was not clear, and the mesenteric vessels were not displayed. The diameter of small arteries in GQDs group was greater and that in the barium meal group was smaller than that before gavage. GQDs, CdTe QDs and barium meal had no notable impact on mouse mesenteric venules. The velocities of blood flow in mesenteric arterioles, venules and capillaries were increased after the administration of barium meal or CdTe QDs, and there were no significant changes in GQDs group. Conclusion GQDs is superior to barium meal in image formation of mouse intestinal wall, and it has less influence on mouse mesenteric microcirculation.
ABSTRACT
<p><b>OBJECTIVE</b>This study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos.</p><p><b>METHODS</b>The 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated.</p><p><b>RESULTS</b>Statistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials.</p><p><b>CONCLUSION</b>MWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.</p>
Subject(s)
Animals , Female , Male , Embryo, Nonmammalian , Embryonic Development , Graphite , Toxicity , Heart Rate , Movement , Nanotubes, Carbon , Toxicity , Oxides , Toxicity , Toxicity Tests , ZebrafishABSTRACT
<p><b>BACKGROUND</b>This study evaluated the feasibility, efficacy and safety of CT-guided percutaneous radiofrequency ablation in patients with spinal osteoid osteoma.</p><p><b>METHODS</b>Two patients suffered spinal osteoid osteoma were treated with CT-guided percutaneous radiofrequency ablation under local anesthesia. Lesions located in sacral vertebrae and cervical vertebrae, which were adjacent to nerve root and spinal canal respectively. Tumors were treated under 90°C radiofrequency temperature lasting 4 minutes by an electrode placement. Visual analog scale was used to evaluate the pain improvement.</p><p><b>RESULTS</b>No complications were observed pre- and post-operation. Patients recovered to normal activities immediately and achieved complete pain relief in 24 hours. No symptoms were recurrent in 5 months and 4 months follow up. Mild scoliosis has been recovered in case 2.</p><p><b>CONCLUSIONS</b>CT-guided percutaneous radiofrequency ablation of spinal osteoid osteoma is safe, effective and has more clinical benefits. The long-term outcome needs further observation.</p>